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Objective: The Huanghuai (HH), which is made from the dried roots of Scutellaria baicalensis (Huangqin in Chinese) and the dried flowers and buds of Sophora japonica (Huaihua in Chinese), is a traditional Chinese formula used to treat dysfunctional uterine bleeding (DUB) (Benglou in Chinese) and proven to treat hemostasis effectively in our previous study. Network pharmacology and molecule docking were performed to study the underlying mechanism of Huanghuai (HH), and pharmacodynamic experiments were conducted to verify its curative effect. Methods: TCMSP, UniProt, GeneCards, STRING, DAVID databases, and Cytoscape 3.7.2 were utilized for the construction of a compound-target-pathway network. Docking the potential effective components with potential targets. The HPLC analysis of the potential effective components was performed. In vivo, the hot plate test model was used to study the analgesic activity, the egg white was used to study the swollen reaction in the sole in mice, and the hemostasis effect was studied by the capillary method, tail-breaking method and abortion uterus test. Results: The results showed that six compounds (acacetin, beta-sitosterol, wogonin, baicalein, kaempferol and quercetin) and four potential targets (PTGS2, AKT1, TP53 and TNF) in the compound-target-pathway network were the potential material basis for HH to treat DUB. It can be seen that the binding energy of the acacetin, wogonin, baicalein, beta-sitosterol, kaempferol and quercetin in HH docked with the receptor proteins PTGS2, AKT1, TP53, and TNF were far less than −5.0 kJ/mol, which means the molecules have low conformational energy, stable structure and high binding activity. And the result of HPLC analysis showed that acacetin, wogonin, baicalein, kaempferol and quercetin were the potential effective components of the hemostasis mechanism of HH, beta-sitosterol was removed due to low content. In vivo testing of the potential effective components, it revealed that the group of potential effective components identified by HPLC could increase the pain threshold, inhibit the swelling hind paws of mice induced by egg white, reduce the bleeding time and clotting time, reduce uterine bleeding, decrease the uterine weight, increase the content of Ca and ET-1, and reduce the content of NO in uterine homogenate tissue, and decrease of E
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Generalized Estimating Equation (GEE) deals with such data that do not have normal distribution in case of repeated experiment and has better properties as compared to rANOVA. With the objective to describe the use of GEE in pharmacological study this endeavour started. GEE described hereby using rat data. Four correlation structured were taken in GEE. It was found Independent/Exchangeable structure best suited with data. The model fit on data was assessed graphically as well. The trend line of repeated data for all cases (rats) were fallen in 95 % bound of predicted model. The model gave the average weight of rat 226.51 gram with start of experiment and it increases 8.67gram per week after feeding high fat diet.
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Chinese medicinal formulae are the important means of clinical treatment in traditional Chinese medicine. It is urgent to use modern advanced scientific and technological means to reveal the complicated mechanism of Chinese medicinal formulae because they have the function characteristics of multiple components, multiple targets and integrated regulation. The systematic and comprehensive research model of proteomic is in line with the function characteristics of Chinese medicinal formulae, and proteomic has been widely used in the study of pharmacological mechanism of Chinese medicinal formulae. The recent applications of proteomic in pharmacological study of Chinese medicinal formulae in anti-cardiovascular and cerebrovascular diseases, anti-liver disease, antidiabetic, anticancer, anti-rheumatoid arthritis and other diseases were reviewed in this paper, and then the future development direction of proteomic in pharmacological study of Chinese medicinal formulae was put forward. This review is to provide the ideas and method for proteomic research on function mechanism of Chinese medicinal formulae.
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A large number of literatures at home and abroad in recent years about Schisandrin B were viewed and its pharmacological effects were summarized. Schisandrin B has a variety of pharmacological activities, which can reduce transaminase activity in liver cells, inhibit lipid peroxidation, anti-oxidant, hepatoprotection, anti-tumor and so on. Its pharmacological effects are accurate and worthy of research and development as a potential drug.
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This study was aimed to investigate the diuretic effect ofMori Cortex and to identify effective fractions inM. Cortex. Metabolic cages were used to firstly observe the diuretic effect ofM. Cortex aqueous extracts on rats. Medications were continuously given for 5 days to screen for the best dosage of diuretic effect. Picric acid assays were used to detect creatinine levels in serum and urine after 5-day medication. Then, the diuretic effect of each chemical split fraction inM. Cortex was studied in order to indentify the effective parts. The results showed that after administration ofM. Cortex aqueous extracts for 3 to 5 days, compared with the control group, there was a significant diuretic effect on rats (P<0.05 orP<0.01). And the medium-dose ofM. Cortex aqueous extracts showed the best effect (P<0.01). However,M. Cortex aqueous extracts had no significant effect on creatinine levels in serum and urine. Assays of diuretic effect of chemical split fractions ofM.Cortex indicated that compared with the control group, 30% ethanol fraction and fatty oil fraction had the best diuretic effect (P<0.01). It was concluded thatM.Cortex aqueous extracts had a significant diuretic effect. And the chemical fractions contributed mostly to this effect were mainly existed in the 30% ethanol fraction and fatty oil fraction.
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The identification of privet in the middle southern United States can be difficult. Because most introduced species of privet can be invasive, and recent mapping projects seek location and species population data, proper identification is important. Ligustrum is currently marketed as a treatment for strengthening the immune system, R 3\05\ 9and on this basis is often recommended for use by people undergoing treatment for cancer or HIV. There is no meaningful scientific evidence that ligustrum provides any benefit for these, or any other, conditions. Very weak evidence from test-tube and animal studies hints that ligustrum might have anti-parasitic, 1 anti-viral, 2l iver-protective , 3immunomodulatory (this means “altering” immune function, rather than, as commonly misunderstood, “strengthening” it), 4,5 and cancer-preventive 6 effects. However, this evidence is too preliminary to rely upon at all. Ligustrum ovalifolium are used the activities of hypotensive activity, hypoglycemic activity, anti inflammatory activity and antioxidant activity.
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Scutellarin (SCU), the main bioactive component of , has been shown to offer beneficial effects on cardiovascular and cerebrovascular functions. SCU effects on angiogenesis and cancer cells and endothelial cells, and it also shows the effect on oxidative stress-induced cell apoptosis. SCU inhibits the translocation of PKC in vivo and in vitro,and may have value as a drug in the treatment of diabetic complications. We reviewed SCU pharmacological research progress in the present paper.
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Dr. Sakaguchi, who has succeeded to the “Asada-style” of Dr. Hosono's Kampo Medicine, has been long studied the dietary theories of Sakurazawa, acupuncture, moxibustion, and homeopathy based on Hosono Kampo Medicine.<br>Dr. Sakaguchi has made great contributions, along with Dr. Shiro Hosono, the reconstruction of Showa Era (1926-1989) Kampo medicine through pharmacological studies of Kampo formulae, and development of their extracts. One remarkable characteristic of Dr. Sakaguchi, as a clinician, is that he attaches importance to “the treatment of minus one-year-olds”, i. e. the treatment by Kampo medicine for a pregnant woman.
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OBJECTIVE:To study the antihypertensive effect of the alcohol soluble composition of Wudiangui.METHODS:The active component in Wudiangui was extracted with 90% alcohol at 40 ℃;with isolated aortic smooth muscle strip of rabbits as experimental material to observe the effects of the alcohol soluble composition of Wudiangui on the dose-response curves of norepinephrine(NE) and Potassium Chloride(KCl) as well as ?-and ? receptors.RESULTS:The alcohol soluble composition of Wudiangui has potent vasodilating effect on rabbits' aortic smooth muscles ex vivo.CONCLUSION:The aortic smooth muscle strips that had been contracted by NE and KCL can be relaxed by the alcohol soluble composition of Wudiangui through blocking calcium channel,and this effect is independent of the blockage of ?-and ? receptors.
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This experiment showed that Yiqipingchuan Pills possessed obvious treatment action of cough and asthma,and expectorant action.It could cause a striking increase in the immunity-organ weight and carbon-particle clearance of hypoimmune mice.It was also found that these pills could make the con- centration of serum-hydrocortisone rise and the Vitamin C content in suprarene lower remarkably.
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Objective: To study the protective effect of Xiaoyao Tablet(XYT)(Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Atractylodis Macrocephalae, Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Herba Menthae, Roasted ginger) on liver. Methods: Using the acute hepatic injury model induced by Tetrachloride and D-galactosamine (CD-GaIN) in rats or mice, we observed the influence of XYT on ALT and AST level in the serum, MDA level and glutathione-s-transferase(GST) activity in the liver homogenates. The pain-killing effect was tested by body twisting model induced by glacial acetic acid and by hot plate model in mice. Results: The XYT reduced the serum ALT level significantly in the acute hepatic injury model in rats and mice. In the CCl 4 reduced acute hepatic injury mice model, XYT decreased the MDA level and increased the GST activity in liver significantly, also it reduced the MDA level in the serum. The XYT reduced twisting number in mice induced by glacial acetic acid. In the hot plate test, the pain threshold was increased. Conclusion: The drug has the similar hepatoprotective function to Xiaoyao Pills.
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Xiaoerzhixie Tablets, composed of many species of Chinese herb medicines, is a compound preparation fined by modern scientific method. it possesses the effects of invigorating spleen and promoting diuresis, regulating stomach-energy, invigorating vital energy, stopping loose bowels and anti-inflam-mation. In the pharmaclolgical study, this Chinse papent medicine was found thai inhibition of anto nomic motion of strips of fundus ventriculi and pieces of intestine separated from rat and guinea pig was obvious. The study also revealed its striking antagonism and bloeking effects on the spastic contraction of duodenum, ileum and colon, induced by acetyleholine, barium chloride and histamine. Not only can it reduce the gastrointestinal drive function but remarkablely raise the phagocytosis of macrophage in body.
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Xingnaojing injection (XNJ) is a mixture extracted and refined from Chinese materia mediea by scientific methods. In this study, the action of XNJ on central nervous system was experimentally evaluated by its effects on free activity of mice, stryehine-induced convulsion, sedation resulting from pentobarbital and respiratory inhibition caused by morphine. The results show that XNJ exhibits biphasie effect on central nervous system, i. e. in small dose, it is a stimulant, while in large dose, an inhibitor.